Abstract: OBJECTIVE: To analyze fingerprint features of low abundant plasma proteome specific to early stage cervical squamous cell carcinoma in Uighur women by two dimensional liquid phase chromatography，for establishment of a plasma proteomic model to distinguish patients with early cancer from the normal. METHODS：We collected 48 plasma samples from Uighur women including 26 cases of early stage cancer at clinical stageⅠ-Ⅱa and 22 cases of cervicitis.After preparation of low abundant plasma proteome by depletion of high abundant plasma proteins，the proteomic fingerprint features of patients with early stage cancer and cervicitis were analysed by separation and analysis of the plasma proteome with two dimensional liquid phase chromatography and subsequent data processing with professional softwares，to establish a differentiating model of plasma proteome for patients with early stage cancer. RESULTS：By setting up the difference of protein content of two or more as a standard for quantitative differences，we found up to 8 peaks with increased and 1 with reduced protein content in patients with early stage of cancer compared to cervicitis. CONCLUSION：The features of low abundant plasma proteome specific to patients with early stage cancer were established. The two-dimensional liquid phase chromatography as high throughput method has a high potential in the research of plasma proteomics. The marked differences in low abundant plasma proteomes between early stage cervical cancer and cervicitis in Uighur women may provide important evidence for the identification of plasma protein markers of the cancer development and early diagnosis in the future.

Key words: Uighur women, cervical squamous cell carcinoma, proteome, 2-D liquid phase chromatography